During the Edo period of Japan out of the fear of losing authority the Tokugawa shogunate hires the Shinsengumi to bring down the Imperial Court However the Satsuma Clan finds out about the Tokugawas scheme and sends the last five Kunoichi female ninjas of Hiryu Ninja Clan to spy on the Shinsengumi Meanwhile an evil skeletal demon is brought to life to murder the Kunoichi ninjas and destroy the world